Coding

Part:BBa_K3619008:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-13)


cDNA encoding the antifungal peptide MsrA2.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for expression in E.coli K12.


Source

MsrA2 is a derivative from the antimicrobial peptide Dermaseptin B1, a cationic peptide found in skin secretion of Phyllomedusa bicolor, arboreal frog. Sequence was obtained through back translation of the peptide in [1].

References

1. https://link.springer.com/article/10.1007/s00122-005-2056-y?shared-article-renderer